Journal
/
/
Introduksjon Multiparametrisk cellulær analyse av Micro Organic Charge-modulerte transistorkjeder med felteffekt
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
In Vitro Multiparametric Cellular Analysis by Micro Organic Charge-modulated Field-effect Transistor Arrays
DOI:

10:05 min

September 20, 2021

,

Capítulos

  • 00:04Introduction
  • 01:12Substrate Selection, Preparation, and Floating Gates (FG): Titanium Deposition
  • 02:11FG Patterning and Gate Dielectric Deposition
  • 03:55Opening of the Sensing Areas of the OCMFET for Electrical Activity Recording and Formation of the Vias to Access the Back of the FGs
  • 04:46Self-Alignment of Source and Drain with the FG
  • 05:15Gold Deposition, Channel Formation, and Patterning of the Sources, Drains, and Control Gates
  • 06:50Deposition and Activation of the Parylene C for pH Sensing
  • 07:57Semiconductor Deposition, Culturing Chamber Placing, and Final Cut-Out of the Device from the PET
  • 08:22Results: Cellular Electrical and Metabolic Activity Recordings with Micro OCMFET Arrays (MOA)
  • 09:27Conclusion

Summary

Tadução automática

Her presenterer vi fabrikasjonsprotokollen til en organisk ladningsmodulert felteffekttransistor (OCMFET)-basert enhet for in vitro cellulær grensesnitt. Enheten, kalt en mikro OCMFET-matrise, er en fleksibel, rimelig og referansefri enhet, som vil muliggjøre overvåking av de elektriske og metabolske aktivitetene til elektroaktive cellekulturer.

Vídeos Relacionados

Read Article