/
/
In situ Hybridisatie in zebravislarven en juvenielen tijdens mesonephrosontwikkeling
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 00:32Raising Larvae
- 01:03Days 1-2: Fixing Larvae and Day 3: Measuring Larvae
- 02:16Days 3 4: Dehydration and Day 5: Rehydration, Proteinase K Digestion, Bleaching and Prehybridization
- 04:05Day 6: Probe Hybridization and Day 7: Probe Washing and Blocking
- 05:38Days 8 9: Antibody Incubation and Days 10 11: Antibody Washing
- 06:05Day 12: Staining and Imaging
- 07:18Results: In Situ Hybridization for Gene Expression Analysis in Zebrafish Larvae and Juveniles During Mesonephros Development
- 07:52Conclusion
