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TDP-43在斑马鱼幼虫脊髓运动神经元中的光遗传学相变

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

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TDP-43在斑马鱼幼虫脊髓运动神经元中的光遗传学相变

DOI:

10.3791/62932-v

•

07:14 min

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February 25, 2022

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Kazuhide Asakawa, Hiroshi Handa, Koichi Kawakami³

¹Department of Chemical Biology,Tokyo Medical University, ²Division of Molecular and Developmental Biology,National Institute of Genetics, ³Department of Genetics,Graduate University for Advanced Studies (SOKENDAI)

Capítulos

00:04Introduction
01:36Preparation of Light Emitting Diode (LED) for Blue Light Illumination
02:13Imaging of Zebrafish Larvae Expressing Optogenetic TAR DNA-binding Protein 43 (TDP-43)
03:58Light Stimulation of Optogenetic TDP-43h-Expressing Fish by Field Illumination of a Blue LED Light
04:35Results: Characterization of TDP-43 Phase Transition in Spinal Motor Neurons of Zebrafish Larvae
06:34Conclusion

Summary

Tadução automática

我们描述了一种以斑马鱼为模型的模式，通过光在脊髓运动神经元中诱导TAR DNA结合蛋白43（TDP-43）的相变。

Tags

Keywords: Optogenetic TDP-43 Spinal Motor Neurons Zebrafish Larvae ALS Phase Transition Protein Aggregation Intrinsically Disordered Regions BAC Transgenesis LED Optical Power Meter Agarose Anesthesia

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