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Alloptisk mekanobiologi avhør av ja-assosiert protein i human kreft og normale celler ved hjelp av et multifunksjonelt system
This content is Free Access.
1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering,University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering,University of Florida, 3Department of Electrical and Computer Engineering,University of California, 4Department of Radiation Oncology, College of Medicine,University of Florida, 5UF Health Cancer Center,University of Florida, 6Department of Physics, College of Liberal Arts and Sciences,University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine,University of Florida
Capítulos
- 00:04Introduction
- 00:53Cell Imaging
- 07:21Results: All-Optical Mechanobiology Interrogation of Yes-Associated Protein in Human Cancer and Normal Cells
- 09:11Conclusion
Dette dokumentet presenterer en detaljert trinnvis protokoll om hvordan du bruker et integrert multifunksjonelt og brukerprogrammerbart system som muliggjør automatisk flerkanals avbildning og mekanobiologisk analyse for å belyse mekano-følsomheten til Ja-assosiert protein (YAP).
Tags
MechanobiologyYes-associated ProteinCancer CellsNormal CellsMulti-functional SystemOptical ElectrophysiologySuper-resolution ImagingLive-cell ImagingHigh-throughputSelf-drift CorrectingLong-term ImagingFluorescent MicroscopyNYCOMEnvironment ChamberMotorized StageCO2 FlowTemperature ControlGlass-bottom Petri DishConfocal ControllerMicroManagerAdhesive TapeAdhesive GlueIntelliJAMFIP
