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Interrogatorio de mecanobiología totalmenteóptica de proteína asociada a sí en cáncer humano y células normales utilizando un sistema multifuncional…
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Bioengineering
Bioengineering
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Interrogatorio de mecanobiología totalmente óptica de proteína asociada a sí en cáncer humano y células normales utilizando un sistema multifuncional
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Article
DOI:
10.3791/62934-v
•
09:55 min
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December 20th, 2021
Interrogatorio de mecanobiología totalmente óptica de proteína asociada a sí en cáncer humano y células normales utilizando un sistema multifuncional
December 20th, 2021
•
Qin Luo*1, Miao Huang*2, Chenyu Liang2, Justin Zhang3, Gaoming Lin1, Sydney Yu1, Mai Tanaka4,5, Sharon Lepler4,5, Juan Guan5,6,7, Dietmar Siemann4,5, Xin Tang2,5
1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 3Department of Electrical and Computer Engineering, University of California, 4Department of Radiation Oncology, College of Medicine, University of Florida, 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida
* These authors contributed equally
Capítulos
Resumo
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Este documento presenta un protocolo detallado sobre cómo utilizar un sistema integrado multifuncional y programable por el usuario que permite la obtención automática de imágenes multicanal y el análisis mecanobiológico para dilucidar la mecanosensibilidad de la proteína asociada al Sí (YAP).