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Çok Fonksiyonlu Bir Sistem Kullanarak İnsan Kanseri ve Normal Hücrelerde Evet İlişkili Proteinin Tamamen Optik Mekanobiyoloji Sorgulaması
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1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering,University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering,University of Florida, 3Department of Electrical and Computer Engineering,University of California, 4Department of Radiation Oncology, College of Medicine,University of Florida, 5UF Health Cancer Center,University of Florida, 6Department of Physics, College of Liberal Arts and Sciences,University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine,University of Florida
Capítulos
- 00:04Introduction
- 00:53Cell Imaging
- 07:21Results: All-Optical Mechanobiology Interrogation of Yes-Associated Protein in Human Cancer and Normal Cells
- 09:11Conclusion
Bu makalede, Otomatik çok kanallı görüntüleme ve mekanobiyolojik analizin Evet ilişkili proteinin (YAP) mekano duyarlılığını aydınlatmasını sağlayan entegre çok fonksiyonlu ve kullanıcı tarafından programlanabilir bir sistemin nasıl kullanılabileceğine dair ayrıntılı bir adım adım protokol sunulmaktadır.
Tags
MechanobiologyYes-associated ProteinCancer CellsNormal CellsMulti-functional SystemOptical ElectrophysiologySuper-resolution ImagingLive-cell ImagingHigh-throughputSelf-drift CorrectingLong-term ImagingFluorescent MicroscopyNYCOMEnvironment ChamberMotorized StageCO2 FlowTemperature ControlGlass-bottom Petri DishConfocal ControllerMicroManagerAdhesive TapeAdhesive GlueIntelliJAMFIP
