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Immunology and Infection

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JoVE Journal Immunology and Infection
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
 

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Article DOI: 10.3791/62937-v • 09:47 min • August 13th, 2021
August 13th, 2021 •
1, 1, 2, 1
1Department of Biology, San Diego State University, 2Institut de Biologie de l’École Normale Supérieure

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Wild Caenorhabditis nematodes are associated with many microbes, often in the gut lumen or infecting the intestine. This protocol details a method to enrich unculturable microbes colonizing the intestine, taking advantage of the resistance of the dauer cuticle.

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Keywords: Caenorhabditis Nematodes Microbiome Enrichment Intestinal Microbiome Unculturable Microbes OP51 Escherichia Coli Dauer Stage M9 Minimal Salts Media Triton X-100 Antibiotic SDS Solution
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