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Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
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Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

DOI:
10.3791/62940-v

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08:30 min

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July 12, 2021

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, , , , , ,
1Department of Reproductive Endocrinology, Wagistrasse 14,University of Zurich and University Hospital Zurich, 2Department of Dermatology, Wagistrasse 18,University of Zurich and University Hospital Zurich, 3Department of Pharmacology and Chemical Biology,University of Pittsburgh

Capítulos

  • 00:04Introduction
  • 00:49Spheroid Formation
  • 03:55Spheroid Immunohistochemistry Study
  • 05:24Live/Dead Staining in Spheroids
  • 05:56Results: Generation of Mammary Epithelial and Endothelial Cell Spheroids
  • 07:41Conclusion

Summary

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Tadução automática

Crosstalk between mammary epithelial cells and endothelial cells importantly contributes to breast cancer progression, tumor growth, and metastasis. In this study, spheroids have been made from breast cancer cells together with vascular and/or lymphatic endothelial cells and demonstrate their applicability as an in vitro system for breast cancer research.

Tags

Breast CancerCell Spheroids3D ModelMammary Epithelial CellsEndothelial CellsCancer Cell-endothelial Cell InteractionsProliferationMetastasisIn Vitro ModelCell DistributionDye StainingEnzymatic DetachmentCell CountingCell SuspensionCell Co-culture

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