Journal
/
Bioengenharia
/
Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids
JoVE Journal
Bioengenharia
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioengenharia
Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

Microfluidic Fabrication of Core-Shell Microcapsules carrying Human Pluripotent Stem Cell Spheroids

DOI:
10.3791/62944-v

•

10:51 min

•

October 13, 2021

•

, , , ,
1Department of Physiology and Biomedical Engineering,Mayo Clinic

Capítulos

  • 00:04Introduction
  • 01:31Solution Preparation
  • 04:21Experimental Setup
  • 07:05hPSC Culture and Analysis in Microcapsules
  • 08:31Results: Analysis of Characterization of Microcapsules and Encapsulation of hPSCs
  • 09:47Conclusion

Summary

Tadução automática

Tadução automática

This article describes encapsulation of human pluripotent stem cells (hPSCs) using a co-axial flow focusing device. We demonstrate that this microfluidic encapsulation technology enables efficient formation of hPSC spheroids.

Tags

Keywords: Microfluidic FabricationCore-shell MicrocapsulesHuman Pluripotent Stem Cell Spheroids3D Cell CultureStem Cell EncapsulationScalable Stem Cell CultivationShear ProtectionCost-effective Cellular TherapyPEGDensity Gradient MediaMineral OilSurfactantDTTTEAPF127Cross-linker EmulsionShielding OilPEG4 Maleimide

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/62944?article_type=v

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
check_url/pt/62944?article_type=v

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
check_url/pt/62944?article_type=v

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices
check_url/pt/62944?article_type=v

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions
check_url/pt/62944?article_type=v

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips
check_url/pt/62944?article_type=v

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
check_url/pt/62944?article_type=v

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
check_url/pt/62944?article_type=v

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
check_url/pt/62944?article_type=v

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting
check_url/pt/62944?article_type=v

Isogenic Kidney Glomerulus Chip Engineered from Human Induced Pluripotent Stem Cells

Read Article