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Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures
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Neurociência
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JoVE Journal Neurociência
Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures

Magnetic Isolation of Microglial Cells from Neonate Mouse for Primary Cell Cultures

DOI:
10.3791/62964-v

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07:23 min

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July 25, 2022

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, , , , , , , , , ,
1NeuroDiderot, Inserm UMR-1141, Hôpital Robert Debré 48,Université de Paris, 2Department of anesthesia and critical care,APHP-Sorbonne university

Capítulos

  • 00:05Introduction
  • 00:32Brain Dissection
  • 01:29Brain Dissociation and Magnetic Microglial Isolation
  • 04:23Phagocytic Assay
  • 05:03Results: Phagocytic Activity and Magnetic Isolation of Microglia In Vitro
  • 06:49Conclusion

Summary

Tadução automática

Tadução automática

Primary microglia cultures are commonly used to evaluate new anti-inflammatory molecules. The present protocol describes a reproducible and relevant method to magnetically isolate microglia from neonate pups.

Tags

Keywords: MicrogliaMagnetic Cell SortingNeonate MousePrimary Cell CultureCD11bCell DissociationHBSSSorting BufferCell SeparationCell Count

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