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Evaluering af leverglukoseproduktion i en polycystisk ovarie syndrom musemodel
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JoVE Journal Medicina
Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Evaluering af leverglukoseproduktion i en polycystisk ovarie syndrom musemodel

DOI:
10.3791/62991-v

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09:44 min

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March 05, 2022

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, , , ,
1Department of Reproductive Endocrinology and Infertility,Baylor College of Medicine, 2Family Fertility Center,Texas Children’s Hospital, 3Children’s Nutrition Research Center,Baylor College of Medicine, 4Critical Care Medicine, Department of Pediatrics,Baylor College of Medicine

Capítulos

  • 00:04Introduction
  • 01:00Preparation of [6,6-2H2]glucose
  • 02:15Set-up of Infusion Experiments
  • 03:19Catheter Insertion
  • 04:24Infusion Set-up and First Infusion
  • 05:51Blood Sampling
  • 06:30Second Infusion
  • 07:13Analyses and Calculations
  • 08:07Resultados
  • 08:39Conclusion

Summary

Tadução automática

Tadução automática

Denne undersøgelse beskriver den direkte måling af leverglukoseproduktion i en polycystisk ovarie syndrom musemodel ved hjælp af en stabil isotop glukose tracer via hale vene i både faste og glukose-rige stater i tandem.

Tags

Hepatic Glucose ProductionPolycystic Ovary SyndromePCOSStable Isotopic Glucose TracerGas Chromatography-mass SpectrometryGCMSFastingGlucose-rich ConditionInfusionC57BL6J Mice

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