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Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
 

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Article DOI: 10.3791/62995-v • 06:32 min • April 13th, 2022
April 13th, 2022 •
1, 1,2
1Department of Internal Medicine, University of Texas Southwestern Medical Center, 2Charles and Jane Pak Center for Mineral Metabolism and Clinical Research, University of Texas Southwestern Medical Center

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This protocol presents a radiolabeled amino acid uptake assay, which is useful for evaluating amino acid consumption either in primary cells or in isolated bones.

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Keywords: Amino Acid Uptake Bone Cells Bone Shafts Radiolabeled Amino Acids Osteoblast Cell Culture ST2 Cells Krebs-Ringer's HEPES Tritiated Glutamine Sodium Dodecyl Sulfate Scintillation Counter Cell Counting Bone Marrow Decellularization
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