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Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
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Neurociência
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JoVE Journal Neurociência
Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

DOI:
10.3791/63007-v

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07:14 min

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October 21, 2021

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, , , , ,
1Division of Radiation Health,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Capítulos

  • 00:04Introduction
  • 00:50Perfusion
  • 02:01Dissection
  • 03:11Adult Brain Dissociation
  • 04:15Debris Removal
  • 05:28Live and Dead Staining
  • 06:00Results: Flow Cytometry Analysis Following Automated Mechanical Dissociation and Enzymatic Digestion of Mouse Brain Hippocampal Tissue
  • 06:51Conclusion

Summary

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This neural cell dissociation protocol is intended for samples with a low amount of starting material and yields a highly viable single-cell suspension for downstream analysis, with optional fixation and staining steps.

Tags

Neural DissociationSingle-cell SuspensionMouse BrainHippocampusEnzymatic DissociationMechanical DissociationPerfusionBrain ExtractionSample Preparation

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