/

/

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

JoVE Journal
Medicina

This content is Free Access.

JoVE Journal Medicina

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

DOI:

10.3791/63009-v

•

04:35 min

•

October 07, 2021

•

Daiki Seya, Yuqing Xu³, Toshifumi Mukunoki, Kenichi Tsujita, Osamu Nakagawa, Yuichiro Arima^2,3,4

¹Department of Molecular Physiology,National Cerebral and Cardiovascular Center Research Institute, ²International Research Center for Medical Sciences (IRCMS),Kumamoto University, ³Department of Cardiovascular Medicine, Graduate School of Medical Sciences,Kumamoto University, ⁴X-Earth Center, Faculty of Advanced Science and Technology,Kumamoto University

Capítulos

00:04Introduction
00:46Perfusion
02:56Results: 3D Evaluation of Murine Blood Vessels in The Lower Extremities of the Mouse
03:58Conclusion

Summary

Tadução automática

Here, we describe a visualization and quantification method for murine hind-limb vessels using micro-X-ray computed tomography.

Tags

Keywords: Computed Tomography 3D Visualization Murine Hind-limb Vessels Sample Preparation Heparin Vasodilation Buffer Paraformaldehyde Contrast Medium Perfusion Hind-limb Ischemia Model Femoral Artery Femoral Vein Collateral Vessels

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Longitudinal Evaluation of Mouse Hind Limb Bone Loss After Spinal Cord Injury using Novel, in vivo, Methodology

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

A Modified Heterotopic Swine Hind Limb Transplant Model for Translational Vascularized Composite Allotransplantation (VCA) Research

Three-dimensional Co-culture Model for Tumor-stromal Interaction

Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Orthotopic Hind Limb Transplantation in the Mouse

Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

Cone Beam Intraoperative Computed Tomography-based Image Guidance for Minimally Invasive Transforaminal Interbody Fusion

Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits

High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model

Read Article