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Erzeugung von Transgenics und Knockouts in Strongyloides-Spezies durch Mikroinjektion
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
DOI:

09:42 min

October 07, 2021

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Capítulos

  • 00:05Introduction
  • 00:37Preparation of Microinjection Slides
  • 01:19Collection of Young Adult Strongyloides for Microinjection
  • 03:19Preparing the Microscope and Breaking the Needle
  • 05:27Microinjecting Strongyloides
  • 07:22Collecting and Screening F1 Larvae to Recover Transgenics/Knockouts
  • 08:31Results: Generating Transgenics and Knockouts in Strongyloides Species Using Intragonadal Microinjection
  • 09:04Conclusion

Summary

Tadução automática

Das funktionelle genomische Toolkit für die parasitären Nematoden Strongyloides stercoralis und Strongyloides ratti umfasst Transgenese, CRISPR/Cas9-vermittelte Mutagenese und RNAi. Dieses Protokoll wird zeigen, wie intragonadale Mikroinjektion verwendet werden kann, um Transgene und CRISPR-Komponenten in S. stercoralis und S. ratti einzuführen.

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