Journal
/
Biologia do Desenvolvimento
/
Rapid Isolation of Single Cells from Mouse and Human Teeth
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Rapid Isolation of Single Cells from Mouse and Human Teeth

Rapid Isolation of Single Cells from Mouse and Human Teeth

DOI:
10.3791/63043-v

•

06:09 min

•

October 28, 2021

•

, , ,
1Department of Histology and Embryology, Faculty of Medicine,Masaryk University, 2Department of Molecular Neuroimmunology, Centre for Brain Research,Medical University of Vienna, 3Department of Physiology and Pharmacology,Karolinska Institute

Capítulos

  • 00:05Introduction
  • 01:09Tissue Dissection
  • 02:58Single-Cell Suspension Preparation
  • 04:42Results: Quantification of the Immune Cells
  • 05:24Conclusion

Summary

Tadução automática

Tadução automática

The current protocol presents a fast, efficient, and gentle method for isolating single cells suitable for single-cell RNA-seq analysis from a continuously growing mouse incisor, mouse molar, and human teeth.

Tags

Keywords: Single-cell IsolationMouse TeethHuman TeethCell SuspensionDental PulpMandibleEuthanasiaHBSSTweezersScalpelCell Quality

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/63043?article_type=v

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
check_url/pt/63043?article_type=v

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells
check_url/pt/63043?article_type=v

Isolation and Characterization of Single Cells from Zebrafish Embryos
check_url/pt/63043?article_type=v

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
check_url/pt/63043?article_type=v

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta
check_url/pt/63043?article_type=v

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood
check_url/pt/63043?article_type=v

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/pt/63043?article_type=v

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
check_url/pt/63043?article_type=v

Single-cell Photoconversion in Living Intact Zebrafish
check_url/pt/63043?article_type=v

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Read Article