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Screening af tobaksgenotyper for phytophthora nicotianaeresistens
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Biologia
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JoVE Journal Biologia
Screening of Tobacco Genotypes for Phytophthora nicotianae Resistance

Screening af tobaksgenotyper for phytophthora nicotianaeresistens

DOI:
10.3791/63054-v

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10:10 min

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April 15, 2022

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, , , , , , ,
1Key Laboratory of Tobacco Genetic Improvement and Biotechnology, Tobacco Research Institute,Chinese Academy of Agricultural Sciences, 2Institute of Rural Development,Zhejiang Academy of Agricultural Sciences, 3Yunnan Academy of Tobacco Agricultural Sciences

Capítulos

  • 00:01Introduction
  • 00:17Materials
  • 00:53Planting Tobacco Genotypes for P. nicotianae Resistance Evaluation
  • 02:07Preparation of Oatmeal Agar Medium
  • 03:11Mycelial Cultivation
  • 03:46Preparation of P. nicotianae Zoospore Suspension
  • 04:32Identification of Disease-Resistant Tobacco Varieties
  • 05:36Evaluation of P. nicotianae Infection
  • 06:49Representative Results
  • 09:36Conclusion

Summary

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Tadução automática

Her præsenteres en protokol til effektiv og nøjagtig screening af tobaksgenotyper for Phytophthora nicotianae-resistens hos frøplanter. Dette er en praktisk tilgang til præcisionsavl samt molekylær mekanismeforskning.

Tags

Tobacco GenotypesPhytophthora NicotianaeResistance ScreeningSeedlingsPrecision BreedingMolecular Mechanism ResearchBeinhart1000-1Beinhart1000 BHXiaohuangjin1025 XHJVermiculitePotting SoilHydroponic DevicesHoagland Nutrient SolutionOatmeal Agar MediumMycelial Cultivation

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