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一种持续分离小鼠心肌细胞的简单有效的方法
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Medicina
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JoVE Journal Medicina
A Simple and Effective Method to Consistently Isolate Mouse Cardiomyocytes
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一种持续分离小鼠心肌细胞的简单有效的方法

DOI:
10.3791/63056-v

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06:25 min

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November 11, 2022

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, , ,
1Department of Physiology and Cell Biology,The Ohio State University

Capítulos

  • 00:04Introduction
  • 00:45Manifold Preparation and Cardiomyocyte Isolation Procedure
  • 05:24Results: Isolation of High‐Quality Mouse Cardiomyocytes
  • 05:55Conclusion

Summary

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Tadução automática

心脏病学细胞和分子功能实验的金标准是心肌细胞。本文介绍了对非朗根道夫技术分离小鼠心肌细胞的适应性。

Tags

Cardiomyocyte IsolationMouse HeartLangendorff ApparatusIschemic TimeIn Vivo PerfusionDigestion BufferTemperature-controlled ManifoldSternumAortaVentricleTweezersHemostatGraduate StudentLaboratory Technique

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