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使用生物发光监测小鼠乳腺癌生长和转移集落形成

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Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

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使用生物发光监测小鼠乳腺癌生长和转移集落形成

DOI:

10.3791/63060-v

•

06:52 min

•

November 05, 2021

•

Balakrishnan Solaimuthu, Arata Hayashi, Anees Khatib, Yoav D. Shaul

¹Department of Biochemistry and Molecular Biology, The Institute for Medical Research Israel-Canada,The Hebrew University-Hadassah Medical School

Capítulos

00:04Introduction
00:50Validating In Vitro Luciferase Activity
01:55Injecting Mice with GFP⁺ Luc⁺ Cells
02:50Measuring the Luciferase Levels in GFP⁺ Luc⁺ Mice
04:43Results: Detection of Tumor Development and Metastatic Colonization In Real-Time
06:05Conclusion

Summary

Tadução automática

在这里，我们描述了一种涉及荧光素酶和绿色荧光蛋白在各种乳腺癌细胞系中表达的非侵入性监测方法。该协议提供了一种在小鼠中实时监测肿瘤形成和转移定植的技术。

Tags

Keywords: Bioluminescence Breast Cancer Metastasis Tumor Growth Cell Lines Luciferase In Vivo Imaging Mouse Model Preclinical Studies

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