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体外研究可生物降解种植体材料的直接和间接培养方法

JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia

Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro

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体外研究可生物降解种植体材料的直接和间接培养方法

DOI:

10.3791/63065-v

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14:49 min

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April 15, 2022

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Changlu Xu*¹, Yiqing Chen*¹, Jiajia Lin, Huinan H. Liu^2,3

¹Materials Science and Engineering Program,University of California, Riverside, ²Department of Bioengineering,University of California, Riverside, ³Stem Cell Center,University of California, Riverside

Capítulos

00:07Introduction
02:04Cell Culture Preparation
02:20Harvesting Bone Marrow Derived Mesenchymal Stem Cells (BMSCs) from Rat Weanlings
04:04Sample Preparation and Sterilization
04:25Cell Culture Methods
06:52Postculture Characterization of Cells
10:22Postculture Analyses of Media and Samples
11:55Representative Results
14:06Conclusions

Summary

Tadução automática

我们介绍了直接培养、直接暴露培养和暴露培养三种方法，用于评估可生物降解植入物材料的体外细胞相容性。这些体外方法模仿不同的体内细胞 - 植入物相互作用，可以应用于研究各种可生物降解的材料。

Tags

Keywords: Biodegradable Implants In Vitro Cell Culture Cytocompatibility Cytotoxicity Direct Culture Direct Exposure Culture Exposure Culture Mesenchymal Stem Cells Biomedical Applications

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