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In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures
JoVE Journal
Neurociência
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JoVE Journal Neurociência
In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

DOI:
10.3791/63068-v

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10:45 min

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October 14, 2021

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, ,
1Laboratory of Axon Growth and Regeneration,Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)

Capítulos

  • 00:05Introduction
  • 01:11Embryo Extraction
  • 01:44Ex Utero and In Utero Electroporation
  • 03:35Brain Extraction and Embedding in Agarose
  • 04:39Organotypic Slice Culture
  • 05:43Image Acquisition and Data Analysis
  • 08:29Results: In Situ Axon Growth and Growth Cone Dynamics
  • 09:56Conclusion

Summary

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Tadução automática

This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.

Tags

Keywords: Axon GrowthGrowth Cone DynamicsEx VivoEmbryonic Brain Slice CulturesIn Situ VisualizationDNA DeliveryMouse Embryonic BrainOrganotypic CultureAxon PlasticityTraumatic ConditionsPathological ConditionsElectroporationBrain DissectionSlicingEx Utero ElectroporationIn Utero ElectroporationLateral VentriclePlatinum Tweezer Electrodes

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