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原位急性离体胚胎脑切片培养物中轴突生长和生长锥动力学的可视化

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

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原位急性离体胚胎脑切片培养物中轴突生长和生长锥动力学的可视化

DOI:

10.3791/63068-v

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10:45 min

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October 14, 2021

•

Eissa Alfadil, Frank Bradke, Sebastian Dupraz

¹Laboratory of Axon Growth and Regeneration,Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)

Capítulos

00:05Introduction
01:11Embryo Extraction
01:44Ex Utero and In Utero Electroporation
03:35Brain Extraction and Embedding in Agarose
04:39Organotypic Slice Culture
05:43Image Acquisition and Data Analysis
08:29Results: In Situ Axon Growth and Growth Cone Dynamics
09:56Conclusion

Summary

Tadução automática

该协议展示了一种直接而稳健 的方法来研究原位 轴突生长和生长锥动态。它描述了如何制备离体生理相关的急性脑切片，并提供了用户友好的分析管道。

Tags

Keywords: Axon Growth Growth Cone Dynamics Ex Vivo Embryonic Brain Slice Cultures In Situ Visualization DNA Delivery Mouse Embryonic Brain Organotypic Culture Axon Plasticity Traumatic Conditions Pathological Conditions Electroporation Brain Dissection Slicing Ex Utero Electroporation In Utero Electroporation Lateral Ventricle Platinum Tweezer Electrodes

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