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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

DOI:
10.3791/63072-v

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04:25 min

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October 28, 2021

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, , , , ,
1Longhua Hospital,Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones, Ministry of Education

Capítulos

  • 00:04Introduction
  • 00:50Preparation of Cells
  • 01:41Surgery for Orthotopic Injection of the Osteosarcoma Cells
  • 02:53Results: Development of Orthotopic Osteosarcoma and Lung Metastasis in Mouse Models
  • 03:55Conclusion

Summary

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The present protocol describes intratibia osteosarcoma cell injection to generate mouse models bearing orthotopic osteosarcoma and pulmonary metastasis lesions.

Tags

Intratibial InjectionOsteosarcoma CellsOrthotopic OsteosarcomaLung MetastasisMouse ModelCell CountingCell SuspensionBasement Membrane MatrixTibia InjectionCell Injection

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