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体内熟练运动行为的无线光遗传学控制

JoVE Journal
Neurociência

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JoVE Journal Neurociência

In Vivo Wireless Optogenetic Control of Skilled Motor Behavior

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体内熟练运动行为的无线光遗传学控制

DOI:

10.3791/63082-v

•

07:52 min

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November 22, 2021

•

Diana L. Rodriguez-Munoz, Omar Jaidar, Marcela Palomero-Rivero, Mario A. Arias-Garcia, Gordon W. Arbuthnott, Violeta G. Lopez-Huerta

¹Institute of Cellular Physiology,National University of Mexico, ²Department of Neurosurgery,Stanford University, ³Facultad de Psicologia,National University of Mexico, ⁴Brain Mechanisms for Behaviour Unit,Okinawa Institute of Science and Technology Graduate University

Capítulos

00:04Introduction
00:57Surgical Procedures
04:48Reach-To-Grasp Training
05:25Results: Kinematic Analysis of Reaching Behavior and Optogenetic Activation of Contralateral and Ipsilateral D1 Spiny Projection Neurons (SPNs)
07:10Conclusion

Summary

Tadução automática

本实验方案描述了如何在单个颗粒到达抓取任务中使用无线光遗传学与高速摄像相结合，以表征参与自由移动小鼠中熟练运动行为表现的神经回路。

Tags

Wireless Optogenetics Skilled Motor Behavior High-speed Videography Neurodevelopmental Disorders Neurodegenerative Disorders LED Cannula Microinjections Stereotaxic Apparatus Bregma Lambda

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