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基于显微镜的测定,用于使用SNARE运输研究和分析回收内体
JoVE Journal
Biologia
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JoVE Journal Biologia
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
DOI:

08:51 min

February 12, 2022

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Capítulos

  • 00:06Introduction
  • 01:04Seeding of Mouse Melanocytes on Pre-Treated Coverslips
  • 01:42Transfection of Cells with the STX13 Plasmids
  • 02:39Fixation of the Cells
  • 03:11Immunostaining of the Cells
  • 05:05Results: Quantification of Overlap Between the RE Localized Proteins and Melanosomes
  • 07:25Results: Quantification of Recycling Endosomes’ Tublar Number and Length
  • 07:56Conclusion

Summary

Tadução automática

回收内体是内体管网络的一部分。在这里,我们提出了一种使用GFP-STX13作为细胞器标记来量化回收内体动力学的方法。

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