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Shotgun 蛋白质组学样品处理由开源实验室机器人自动执行

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Shotgun Proteomics Sample Processing Automated by an Open-Source Lab Robot

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Shotgun 蛋白质组学样品处理由开源实验室机器人自动执行

DOI:

10.3791/63092-v

•

10:12 min

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October 28, 2021

•

Yu Han³, Cody T. Thomas³, Sara A. Wennersten³, Edward Lau³, Maggie P. Y. Lam^2,3

¹Department of Medicine-Cardiology,University of Colorado Anschutz Medical Campus, ²Department of Biochemistry & Molecular Genetics,University of Colorado Anschutz Medical Campus, ³Consortium for Fibrosis Research & Translation, School of Medicine,University of Colorado Anschutz Medical Campus

Capítulos

00:04Introduction
00:40Mass Spectrometry (MS) Sample Preparation with a Single Protein
04:44Peptide Clean-up Using SP3 Paramagnetic Beads
06:19MS Sample Preparation with Protein Lysate
08:37Results: Mass Spectrometry Proteomics with a Single Protein Standard Bovine Serum Albumin and a Detergent-Containing Human Heart Lysate Sample
09:44Conclusion

Summary

Tadução automática

提供了详细的协议和三个Python脚本，用于操作开源机器人液体处理系统，以执行质谱实验的半自动蛋白质样品制备，包括去垢剂去除，蛋白质消化和肽脱盐步骤。

Tags

Shotgun Proteomics Automated Sample Processing Open-source Lab Robot Liquid Handling System Mass Spectrometry Protein Biochemistry Proteomic Studies Ammonium Bicarbonate DTT Temperature Control Automation Open-source Python Scripts

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