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小鼠大脑中人类神经祖细胞的脑内移植和 体内 生物发光跟踪
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain
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小鼠大脑中人类神经祖细胞的脑内移植和 体内 生物发光跟踪

DOI:
10.3791/63102-v

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06:12 min

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January 27, 2022

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, , , , , , , ,
1Institute for Regenerative Medicine,University of Zurich, 2Neuroscience Center Zurich,University of Zurich and ETH Zurich

Capítulos

  • 00:05Introduction
  • 00:55Cell Preparation
  • 01:53Transplantation Procedure
  • 02:56Initial Setup of Imaging
  • 04:12Results: Analysis of the Transplanted Cells
  • 05:15Conclusion

Summary

Tadução automática

Tadução automática

我们描述了用小鼠大脑中表达荧光素酶 - 绿色荧光蛋白（GFP）的双报告载体转导的人神经祖细胞的实质内移植。移植后，使用荧光显微镜在脑切片 中鉴定的体内 生物发光和表达GFP的移植细胞反复测量荧光素酶信号。

Tags

Intracerebral TransplantationIn Vivo Bioluminescence TrackingHuman Neural Progenitor CellsMouse BrainCell-based TherapyBrain RegenerationLongitudinal ImagingCell MigrationCell SurvivalLuciferase-expressing Cell LineStereotaxic SurgeryCell PreparationCell TransplantationIn Vivo ImagingBioluminescence ImagingLiving Image Software

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