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Isolation of Epithelial Cells from Human Dental Follicle
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Biologia
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JoVE Journal Biologia
Isolation of Epithelial Cells from Human Dental Follicle

Isolation of Epithelial Cells from Human Dental Follicle

DOI:
10.3791/63104-v

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04:07 min

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November 05, 2021

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1Department of Conservative Dentistry,Kyung Hee University Dental Hospital at Gangdong, 2Department of Conservative Dentistry, School of Dentistry,Kyung Hee University

Capítulos

  • 00:04Introduction
  • 00:34Isolate Single-Cell Populations from Dental Follicle
  • 02:52Results: Emergence of Epithelial Cells
  • 03:13Conclusion

Summary

Tadução automática

Tadução automática

The dental follicle contains an epithelial population and mesenchymal cells. The epithelial population was selected from the heterogeneous dental follicle cell population by providing a distinct culture medium. Epithelial cells survived and formed colonies in a serum-free medium.

Tags

Dental FollicleEpithelial CellsCell IsolationCell CultureCollagenaseProteaseTrypsin-EDTAKeratinocyte MediumCell StrainerCentrifugationCell MorphologyCell Colonies

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