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An Epithelial Abrasion Model for Studying Corneal Wound Healing
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JoVE Journal Medicina
An Epithelial Abrasion Model for Studying Corneal Wound Healing

An Epithelial Abrasion Model for Studying Corneal Wound Healing

DOI:
10.3791/63112-v

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04:45 min

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December 29, 2021

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, , , ,
1College of Optometry,University of Houston, 2Children’s Nutrition Research Center,Baylor College of Medicine, 3Center for Translational Research on Inflammatory Diseases (CTRID),Michael E. DeBakey Veterans Affairs Medical Center

Capítulos

  • 00:05Introduction
  • 00:47Preparation and Anesthesia
  • 01:34Creation of Corneal Wound, Monitoring of Wound Closure, and Re-Epithelialization
  • 03:30Results: Inflammatory Response and Wound Closure Following Central Corneal Epithelial Abrasion
  • 04:18Conclusion

Summary

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Tadução automática

Here, a protocol for creating a central corneal epithelial abrasion wound in the mouse using a trephine and a blunt golf club spud is described. This corneal wound healing model is highly reproducible and is now being used to evaluate compromised corneal wound healing in the context of diseases.

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Epithelial AbrasionCorneal Wound HealingCorneal InflammationCorneal Wounding ModelFluorescein StainingWound ClosureRe-epithelializationImage Analysis

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