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细胞膜亲和色谱柱鉴定与固定化托肌球蛋白激酶受体B相互作用的特化植物代谢物
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Bioquímica
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JoVE Journal Bioquímica
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
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细胞膜亲和色谱柱鉴定与固定化托肌球蛋白激酶受体B相互作用的特化植物代谢物

DOI:
10.3791/63118-v

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11:44 min

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January 19, 2022

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, , , ,
1Department of Biological Sciences,The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy,Hacettepe University

Capítulos

  • 00:05Introduction
  • 00:52Cell Homogenization
  • 03:56Cell Membrane Solubilization
  • 04:46Cell Membrane Immobilization on IAM.PC.DD2 Particles
  • 06:23CMAC Column Packing
  • 08:57Results: Antibody Staining and Frontal Affinity Chromatography of Immobilized TrkB Receptors
  • 11:02Conclusion

Summary

Tadução automática

Tadução automática

该协议描述了用含有功能性跨膜滋养肌球蛋白激酶受体B蛋白的固定化细胞膜片段制备细胞膜亲和色谱（CMAC）柱。还解释了使用CMAC色谱柱鉴定与这些受体相互作用并存在于复杂天然混合物中的特化植物代谢物。

Tags

Keywords: Cellular Membrane Affinity ChromatographyTransmembrane ReceptorsPlant MetabolitesTropomyosin Kinase ReceptorBenzamidinePMSFProtease InhibitorATPTris-HCl BufferHomogenization BufferCell HarvestingCell LysisColumn Preparation

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