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Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection
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Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection
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Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection

DOI:
10.3791/63123-v

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05:49 min

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November 01, 2021

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, , ,
1Department of Experimental and Clinical Medicine, Section of Internal Medicine,University of Florence

Capítulos

  • 00:05Introduction
  • 01:00Mice Preparation for Ultrasound-Guided Injection (US-GI)
  • 02:23Injection of PANC1 Cells in the Pancreas by Ultrasound-Guided Injection (US-GI) Method
  • 04:17Results: Development of Orthotopic Pancreatic Cancer (PCa) Xenograft in the Mouse Pancreas
  • 05:06Conclusion

Summary

Tadução automática

Tadução automática

We present a protocol to generate a minimally invasive orthotopic pancreatic cancer model by ultrasound-guided injection of human pancreatic cancer cells and the subsequent monitoring of tumor growth in vivo by ultrasound imaging.

Tags

Keywords: Orthotopic XenograftPancreatic CancerUltrasound-guided InjectionMouse ModelPANC1 CellsB-Mode ImagingMinimally Invasive3Rs PrinciplesSmall Molecules

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