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从小鼠中分离和培养骨髓衍生树突状细胞的经济有效的方案
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice
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从小鼠中分离和培养骨髓衍生树突状细胞的经济有效的方案

DOI:
10.3791/63125-v

•

04:29 min

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July 01, 2022

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, , , , ,
1Tianjin Institute of Urology,The Second Hospital of Tianjin Medical University, 2Department of Pathology,The Second Hospital of Tianjin Medical University, 3Department of Urology,The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University

Capítulos

  • 00:17Introduction
  • 00:37Isolation of Bone Marrow and Preparation of BM Cells
  • 02:05Induction Culture of BMDC
  • 03:11Resultados
  • 03:59Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了一种经济有效的方法，在用10 ng / mL GM-CSF / IL-4培养7天后从小鼠中分离和产生高纯度骨髓来源的树突状细胞。

Tags

Bone Marrow-derived Dendritic CellsIsolationCultureMouseBMDCCD11cCD80MSC2GM-CSFIL-4Cost-effectiveEfficient

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