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Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
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Biologia
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JoVE Journal Biologia
Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

DOI:
10.3791/63128-v

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09:12 min

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October 04, 2021

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, , , , ,
1Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University, 2Division of Cardiology,Georgia Institute of Technology and Emory University

Capítulos

  • 00:05Introduction
  • 00:51Partial Carotid Ligation (PCL) Surgery
  • 01:57Isolation of Carotid Arteries Post Sacrifice
  • 03:02Endothelial-Cell-Enriched Single-Cell Isolation from Mice Carotids
  • 04:02Flushing the Carotid Arteries
  • 05:53Single-Cell and Single-Nucleus Analyses
  • 07:18Results: Standard QC Metrics for the scRNAseq and scATACseq Study
  • 08:34Conclusion

Summary

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We present a method for isolating endothelial cells and nuclei from the lumen of mouse carotid arteries exposed to stable or disturbed flow conditions to perform single-cell omics experiments.

Tags

Keywords: Endothelial CellsCarotid ArterySingle-cell Multi-omicsAtherosclerosisBlood VesselPerfusionDigestion BufferLigationDissection

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