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Monitoring Gut Acidification in the Adult Drosophila Intestine
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Biologia
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JoVE Journal Biologia
Monitoring Gut Acidification in the Adult Drosophila Intestine

Monitoring Gut Acidification in the Adult Drosophila Intestine

DOI:
10.3791/63141-v

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04:39 min

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October 11, 2021

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,
1Children’s Research Institute,University of Texas Southwestern Medical Center

Capítulos

  • 00:05Introduction
  • 00:58Preparing for the Gut Acidification Monitoring Assay
  • 01:54Gut Acidification Monitoring Assay
  • 02:30Mounting and Image Acquisition
  • 02:52Results: Gut Acidification Monitoring and Phylogeny of Gut Acidification Phenomenon
  • 03:56Conclusion

Summary

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Here, we present a standardized protocol for monitoring gut acidification in Drosophila melanogaster with optimal output. We first use this protocol for gut acidification monitoring in Drosophila melanogaster and then demonstrate its use in non-model Drosophila species.

Tags

Keywords: Gut AcidificationDrosophila IntestineBromophenol BluePH SensingFood PreparationFly DissectionGut IsolationMicroscopyAcidification MonitoringAcidic GutNon-acidic GutPercentage Calculation

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