Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Detection of Plasmodium Sporozoites in Anopheles…
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biology
Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay
 

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

Article DOI: 10.3791/63158-v • 07:04 min • September 30th, 2021
September 30th, 2021 •
1, 2, 3, 1, 4
1Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, 2Department of Molecular Tropical Medicine & Genetics, Faculty of Tropical Medicine, Mahidol University, 3Department of Internal Medicine, Morsani College of Medicine, University of South Florida, 4Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University

Capítulos

Resumo
Automatic Translation

This protocol describes a sandwich enzyme-linked immunosorbent assay to detect salivary gland sporozoites in mosquitoes. Using easily available monoclonal antibodies, the method enables cost-effective, high-throughput detection of mosquitoes carrying Plasmodium falciparum or Plasmodium vivax. The method is suitable for malaria transmission research, including vector surveys.

Tags

Keywords: Plasmodium Sporozoites Anopheles Mosquitoes Enzyme-linked Immunosorbent Assay (ELISA) Plasmodium Circumsporozoite Protein (CSP) Plasmodium Falciparum Plasmodium Vivax Malaria Transmission Mosquito Infectiousness Vector Species
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter