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前子宫 从前肠胚形成到晚期器官生成的小鼠胚胎培养

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

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前子宫从前肠胚形成到晚期器官生成的小鼠胚胎培养

DOI:

10.3791/63160-v

•

07:14 min

•

October 19, 2021

•

Alejandro Aguilera-Castrejon, Jacob H. Hanna

¹Department of Molecular Genetics,Weizmann Institute of Science

Capítulos

00:04Introduction
00:47Ex Utero Roller Culture of Embryos from Embryonic Day 7.5 to 11
03:48Extended Embryo Culture from Embryonic Day 5.5/6.5 to 11
04:38Results: Continuous and Robust Ex Utero Development of Post Implantation Mouse Embryos
06:34Conclusion

Summary

Tadução automática

增强的全胚胎培养平台允许植入后小鼠胚胎的连续和稳健的宫外发育长达六天，从前肠胚形成阶段到高级器官发生。在该协议中，我们详细介绍了使用静态板和旋转瓶系统成功培养胚胎的标准程序。

Tags

Ex Utero Culture Mouse Embryos Pregastrulation Organogenesis Gastrulation Roller Culture Incubator Gas Concentration Gas Pressure Embryo Dissection Culture Medium DPBS Pregnant Mouse

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