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老年人血浆中酚类代谢物的半靶向超高效色谱耦合质谱分析
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Bioquímica
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JoVE Journal Bioquímica
Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
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老年人血浆中酚类代谢物的半靶向超高效色谱耦合质谱分析

DOI:
10.3791/63164-v

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14:39 min

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April 22, 2022

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, , , ,
1Department of Chemical Biological Sciences,Universidad Autónoma de Ciudad Juárez

Capítulos

  • 00:42Introduction
  • 02:58Sample Preparation
  • 04:37UPLC-MS/MS Analysis
  • 06:36Database Construction
  • 09:49Data Analysis
  • 11:25Representative Results
  • 14:13Conclusions

Summary

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该协议的目标是使用半靶向色谱 - 质谱法检测血浆中的酚类代谢物。

Tags

Semi-targetedUltra-high-performance ChromatographyMass SpectrometryPhenolic MetabolitesPlasmaElderly AdultsUPLC-MS/MSProtein PrecipitationMetabolite IdentificationBrosimum AlicastrumBioavailabilityBiotransformationUntargeted AnalysisSemi-targeted AnalysisMolecular FeaturesMass-to-charge RatioIsotopic DistributionFragmentation PatternPolyphenol Metabolites

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