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Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug
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Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

Microsurgical Skills of Establishing Permanent Jugular Vein Cannulation in Rats for Serial Blood Sampling of Orally Administered Drug

DOI:
10.3791/63167-v

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08:28 min

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December 14, 2021

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, , , , ,
1Xi’an Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Medical Research,Northwestern Polytechnical University

Capítulos

  • 00:04Introduction
  • 00:52Jugular Vein Isolation
  • 02:29Jugular Vein Cannulation
  • 04:09Catheter Exteriorization
  • 05:41Physiological and Hematological Monitoring During Recovery Phase
  • 06:41Results: Physiological-Hematological Monitoring and Pharmacokinetics of Ellagic Acid in Jugular Vein Cannulation (JVC) Rat Model
  • 07:48Conclusion

Summary

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Detailed microsurgical techniques are demonstrated to establish a longer-term jugular vein cannulation rat model for sequential blood collection in the same animal. Physiological and hematological parameters have been monitored during the rat's recovery phase. This model has been applied to study pharmacokinetics of orally administered polyphenol without inducing animal stress.

Tags

Jugular Vein CannulationRatMicrosurgeryBlood SamplingPharmaceutical ResearchVein LigationCatheter InsertionHeparinized Saline

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