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小鼠输卵管的显微解剖和解离：个体片段鉴定和单细胞分离

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

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小鼠输卵管的显微解剖和解离：个体片段鉴定和单细胞分离

DOI:

10.3791/63168-v

•

07:44 min

•

November 04, 2021

•

Kelly C. Radecki, Mary Y. Lorenson, David G. Carter, Ameae M. Walker

¹Division of Biomedical Sciences, School of Medicine,University of California, Riverside, ²Microscopy and Imaging Core Facility,University of California, Riverside

Capítulos

00:05Introduction
01:40Macrodissection of The Ovary, Oviduct, and Uterus
03:28Microdissection and Segmentation of the Oviduct
04:15Oviduct Dissociation
04:45Results: Oviduct Cell Dissociation and Quality Assessment of Segmental RNA
06:42Conclusion

Summary

Tadução automática

本文提出了一种小鼠输卵管显微解剖方法，该方法允许在保持RNA完整性的同时收集单个片段。此外，描述了非酶促卵管细胞解离过程。这些方法适用于功能不同的输卵管节段和解离的输卵管细胞的后续基因和蛋白质分析。

Tags

Microdissection Dissociation Murine Oviduct Segment Identification Single Cell Isolation Ampulla Infundibulum Stromal Cells Epithelial Cells Immune Cells Smooth Muscle Cells In Vitro Fertilization Malignant Transformation

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