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1LumiSTAR Biotechnology, Inc., 2NEXEL Co., Ltd., 3Institute of Pharmacology, College of Medicine,National Taiwan University, 4Manchester Heart Centre, Manchester Royal Infirmary,Manchester University NHS Foundation Trust, 5Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre,University of Manchester, 6Division of Cell Matrix Biology and Regenerative Medicine,University of Manchester
Capítulos
- 00:04Introduction
- 00:57iPSC Derived Cardiomyocytes Preparation
- 02:28Expression of Genetically Encoded Calcium Indicators
- 03:24Image Acquisition and Functional Assays for Toxicity Test and Phenotypic Screening
- 05:22Small Molecule Preparation
- 05:58Imaging Processing and Analysis
- 06:31Results: Imaging and Processing of Cells
- 09:28Conclusion
Den nuværende protokol beskriver optisk kontrol og observation af udløst cellulær aktivitet i iPSC-afledte kardiomyocytter (iPSC-CM'er) til screening af lægemidler med høj kapacitet og toksicitetstest. Multi-parametrisk kvantificering af fænotypiske mønstre i tid og rum vises. Langtidsvirkninger af lægemidler over timer eller sekventielle målinger over dage demonstreres.
