/
/
Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Introduction
- 00:48Egg Incubation and Embryo Staging
- 02:08Bead Preparation
- 04:34Embryo Manipulation and Interdigit Implantation
- 05:42Results: Evaluation of Cell Behavior in the Embryonic Chick Limb Using Soaked Beads
- 06:20Conclusion
The soaked bead assay involves targeted delivery of test reagent at any developmental time point to study the regulation of cell differentiation and morphogenesis. A detailed protocol, applicable to any experimental animal model, for preparing three different types of soaked beads and implanting these in the interdigit of a chicken embryo is presented.
