É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:03Introduction
- 01:17Krebs-Henseleit Buffer Preparation
- 01:45Perfusion System Preparation
- 02:29Calibration and Preparation of the NMR Spectrometer for Acquisition
- 03:22Animal Preparation, Surgical Procedure, and Perfusion of the Heart in the NMR Tube
- 09:23Acquiring Data for Cardiac Energetics and pH
- 10:09DNP Spin Polarization and Dissolution
- 11:25Hyperpolarized 13C Spectroscopy
- 12:07Representative Results
- 14:40Conclusion
