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Immunology and Infection

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JoVE Journal Immunology and Infection
Chemical Cartography Approaches to Study Trypanosomatid Infection
 

Chemical Cartography Approaches to Study Trypanosomatid Infection

Article DOI: 10.3791/63255-v • 08:21 min • January 21st, 2022
January 21st, 2022 •
*1,2, *2,3, 1, 1,2,4
1Department of Chemistry and Biochemistry, University of Oklahoma, Norman, 2Laboratories of Molecular Anthropology and Microbiome Research, University of Oklahoma, Norman, 3Department of Anthropology, University of Oklahoma, Norman, 4Department of Microbiology and Plant Biology, University of Oklahoma, Norman
* These authors contributed equally

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This protocol describes the steps to generate a 3D model of metabolite distribution during trypanosomatid infection, including sample collection, metabolite extraction, an overview of liquid chromatography-tandem mass spectrometry data acquisition, 3D model generation, and finally, data visualization.

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Keywords: Chemical Cartography 3D Modeling Metabolite Distribution Parasite Distribution Tissue Metabolic Perturbation Cross-organ Mapping Metabolomics Tissue Homogenization Water-based Homogenization Organic Extraction Mass Spectrometry SketchUp Software Organ Mapping Tissue Sectioning Pathogenesis Trypanosomatid Infection
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