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用于微生物化学和蛋白质生产的光控发酵

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Light-Controlled Fermentations for Microbial Chemical and Protein Production

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用于微生物化学和蛋白质生产的光控发酵

DOI:

10.3791/63269-v

•

08:37 min

•

March 22, 2022

•

Shannon M. Hoffman*¹, Makoto A. Lalwani*¹, José L. Avalos^2,3,4

¹Department of Chemical and Biological Engineering,Princeton University, ²The Andlinger Center for Energy and the Environment,Princeton University, ³Department of Molecular Biology,Princeton University, ⁴High Meadows Environmental Institute,Princeton University

Capítulos

00:04Introduction
00:43Light-Controlled Chemical Production Using the Saccharomyces cerevisiae OptoINVRT7 Circuit
05:12Three-Phase Fermentation Using the Saccharomyces cerevisiae OptoAMP System
06:26Results: The Effects of Light-Regulated Fermentations for Chemical and Protein Production
08:01Conclusion

Summary

Tadução automática

微生物代谢的光遗传学控制为发酵过程提供了灵活的动态控制。这里的实验方案展示了如何建立蓝光调节的发酵，用于不同体积尺度的化学和蛋白质生产。

Tags

Keywords: Optogenetics Light-controlled Fermentation Microbial Chemical Production Microbial Protein Production Saccharomyces Cerevisiae OptoINVRT PIGA1 Promoter Blue Light Genetic Engineering Bioprocessing Biomanufacturing

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