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使用 体内 抑制、免疫荧光和流式细胞术对精子细胞中驱动蛋白-7 CENP-E 进行功能评估
JoVE Journal
Biologia
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JoVE Journal Biologia
Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
DOI:

09:41 min

December 28, 2021

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Capítulos

  • 00:05Introduction
  • 01:13Construction of GSK923295-Mediated Centromere Protein E (CENP-E) Inhibition Mouse Models
  • 02:18Hematoxylin-eosin (HE) Staining and Histopathology
  • 03:57Immunofluorescence and Confocal Microscopy
  • 05:31Flow Cytometry
  • 07:04Results: Analysis of Seminiferous Tubules and the Disruption of Spermatogenesis Post CENP-E Inhibition by GSK923295
  • 08:59Conclusion

Summary

Tadução automática

本文报道了通过腹部手术和睾丸注射GSK923295对CENP-E的 体内 抑制,这是男性减数分裂的宝贵模型。使用免疫荧光,流式细胞术和透射电子显微镜测定,我们发现CENP-E抑制导致小鼠精子细胞中的染色体错位和基因组不稳定。

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