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1Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital,Affiliated Hospital of Fujian Medical University
Capítulos
- 00:05Introduction
- 01:13Construction of GSK923295-Mediated Centromere Protein E (CENP-E) Inhibition Mouse Models
- 02:18Hematoxylin-eosin (HE) Staining and Histopathology
- 03:57Immunofluorescence and Confocal Microscopy
- 05:31Flow Cytometry
- 07:04Results: Analysis of Seminiferous Tubules and the Disruption of Spermatogenesis Post CENP-E Inhibition by GSK923295
- 08:59Conclusion
Cet article rapporte une inhibition in vivo de la CENP-E par chirurgie abdominale et injection testiculaire de GSK923295, un modèle précieux pour la division méiotique masculine. En utilisant les tests d’immunofluorescence, de cytométrie en flux et de microscopie électronique à transmission, nous montrons que l’inhibition de la CENP-E entraîne un désalignement chromosomique et une instabilité du génome dans les spermatocytes de souris.
