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1Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital,Affiliated Hospital of Fujian Medical University
Capítulos
- 00:05Introduction
- 01:13Construction of GSK923295-Mediated Centromere Protein E (CENP-E) Inhibition Mouse Models
- 02:18Hematoxylin-eosin (HE) Staining and Histopathology
- 03:57Immunofluorescence and Confocal Microscopy
- 05:31Flow Cytometry
- 07:04Results: Analysis of Seminiferous Tubules and the Disruption of Spermatogenesis Post CENP-E Inhibition by GSK923295
- 08:59Conclusion
Denne artikkelen rapporterer en in vivo hemming av CENP-E gjennom abdominal kirurgi og testikkelinjeksjon av GSK923295, en verdifull modell for mannlig meiotisk deling. Ved hjelp av immunfluorescens, flowcytometri og transmisjonselektronmikroskopianalyser viser vi at CENP-E-hemming resulterer i kromosomfeilstilling og genominstabilitet i musespermatocytter.
