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蓝藻中完整藻胆体的分离与表征

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

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蓝藻中完整藻胆体的分离与表征

DOI:

10.3791/63272-v

•

06:26 min

•

November 10, 2021

•

Han-Wei Jiang, Ming-Yang Ho²

¹Department of Life Science,National Taiwan University, ²Institute of Plant Biology,National Taiwan University

Capítulos

00:04Introduction
00:38Cell Culture and Harvesting
01:13Cells Lysis
02:43Preparation of the Sucrose Gradient Buffers and PBS Isolation
03:32Measurement of PBS Fluorescence at 77K
04:00Results: Spectral Evaluation of Phycobilisomes
05:50Conclusion

Summary

Tadução automática

目前的方案详细介绍了通过不连续蔗糖密度梯度离心从蓝藻中分离藻胆体的过程。完整的藻糖体的分数通过77K荧光发射光谱和SDS-PAGE分析得到证实。所得的藻胆体组分适用于TEM的负染色和质谱分析。

Tags

Phycobilisome Cyanobacteria Isolation Characterization Protocol B-HEPES Medium Potassium Phosphate Buffer Cell Lysis Triton X-100 Sucrose Gradient Centrifugation

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