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由Baermann Funnel快速分离野生线虫

JoVE Journal
Genética

This content is Free Access.

JoVE Journal Genética

Rapid Isolation of Wild Nematodes by Baermann Funnel

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由Baermann Funnel快速分离野生线虫

DOI:

10.3791/63287-v

•

05:55 min

•

January 31, 2022

•

Sophia C. Tintori*¹, Solomon A. Sloat*¹, Matthew V. Rockman

¹Department of Biology and Center for Genomics & Systems Biology,New York University

Capítulos

00:04Introduction
00:56Collection of the Nematode Substrates
01:43Preparation of an Array of Baermann Funnels
02:21Transferring of Samples into the Funnels
03:23Extraction of Nematodes from the Funnels
04:08Establishing the Cultures
04:45Results: Success of Nematode Isolation by Baermann Funnel
05:18Conclusion

Summary

Tadução automática

该协议概述了一种从现场天然底物中有效提取活线虫的方法。

Tags

Baermann Funnel Wild Nematodes Caenorhabditis Field Collection Substrate Ecology Population Genetics MetaGenetics Quantitative Genetics Sample Preparation Field Research

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