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Visualizing Cytoskeleton-Dependent Trafficking of Lipid-Containing Organelles in Drosophila Embryos
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Capítulos
- 00:05Introduction
- 00:40Injection Needle Preparation
- 01:26Injection Solution Preparation and Needle Loading
- 02:08Embryo Preparation for Microinjection
- 04:34Microinjecting Embryos
- 05:51Imaging Embryos
- 06:38Results: Representative Results for Organelle Dependent Trafficking in Drosophila Embryos
- 08:14Conclusion
In the early Drosophila embryo, many organelles are motile. In principle, they can be imaged live via specific fluorescent probes, but the eggshell prevents direct application to the embryo. This protocol describes how to introduce such probes via microinjection, and then analyze bulk organelle motion via particle image velocimetry.
